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ALAT-2 laser aggregation analyzer
The BIOLA aggregation analyzers are designed to study platelet aggregation, as well as to determine the concentration of cells in suspension and to assess their shape.
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NPF BIOLA LLCDescription
ALAT-2 offers two methods for platelet aggregation research: the turbidimetric method (the Born method) and the Optical Density Fluctuation Analysis (ODFA) method.
The turbidimetric method proposed by Born and O’Brien is the most common method that is used in researching aggregation. By registering the changes in the optical density of platelet-rich plasma it allows investigating not only the aggregation, but also the changes in the shape of cells. The latter is particular important, because the changes in platelet shape can mask the initial stage of aggregation. It has been established that the formation of microaggregates (consisting of less than 100 platelets) may not affect the optical density in a suspension.
The ODFA method proposed by Z.A. and co-authors in 1989 is associated with a change in the average radius of the aggregates and is based on the analysis of light transmission fluctuations caused by a random change in the number of particles in the optical channel. The relative dispersion of such fluctuations is proportional to the average size of the aggregates and is used to study the aggregation kinetics. Remarkably high sensitivity of the ODFA method makes it employable in studying spontaneous aggregation, aggregation under the action of low concentrations of inducers, as well as aggregation of subcellular particles and macromolecules. Further studies by Biola LTD specialists proved possible to evaluate the concentration of particles in stirred suspension.
Field of application
Diagnosis of platelet condition in hemostasis;
Diagnosis of congenital and acquired hemostasis disorders;
Evaluation of the effectiveness of therapy and the degree of impact on the aggregation;
creening new drugs;
Evaluation of the viability of platelet mass during blood transfusion;
Toxicology;
Evaluation of the form and number of platelets;
Quantitative determination of von Willebrand factor;
Aggregation is recorded both by the traditional turbidimetric method and by the method based on real-time measurement of aggregate mean size.
The turbidimetric method proposed by Born and O’Brien is the most common method that is used in researching aggregation. By registering the changes in the optical density of platelet-rich plasma it allows investigating not only the aggregation, but also the changes in the shape of cells. The latter is particular important, because the changes in platelet shape can mask the initial stage of aggregation. It has been established that the formation of microaggregates (consisting of less than 100 platelets) may not affect the optical density in a suspension.
The ODFA method proposed by Z.A. and co-authors in 1989 is associated with a change in the average radius of the aggregates and is based on the analysis of light transmission fluctuations caused by a random change in the number of particles in the optical channel. The relative dispersion of such fluctuations is proportional to the average size of the aggregates and is used to study the aggregation kinetics. Remarkably high sensitivity of the ODFA method makes it employable in studying spontaneous aggregation, aggregation under the action of low concentrations of inducers, as well as aggregation of subcellular particles and macromolecules. Further studies by Biola LTD specialists proved possible to evaluate the concentration of particles in stirred suspension.
Field of application
Diagnosis of platelet condition in hemostasis;
Diagnosis of congenital and acquired hemostasis disorders;
Evaluation of the effectiveness of therapy and the degree of impact on the aggregation;
creening new drugs;
Evaluation of the viability of platelet mass during blood transfusion;
Toxicology;
Evaluation of the form and number of platelets;
Quantitative determination of von Willebrand factor;
Aggregation is recorded both by the traditional turbidimetric method and by the method based on real-time measurement of aggregate mean size.